Article
  • Wound Healing Effect of Gallic Acid-g-Chitosan Gel

  • Jin Kim*, **, Jae-Young Je***,† , and Chang-Moon Lee****, *****, ******,†

  • *Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University, Gwangju 61452, Korea
    **Institute of Dental Science, Chosun University, Gwangju 61452, Korea
    ***Division of Smart Healthcare, Pukyong National University, Busan 48513, Korea
    ****School of Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea
    *****Department of Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea
    ******Research Center of Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea

  • Gallic Acid가 결합된 키토산 젤의 창상 치유 효과

  • 김 진*, ** ·제재영***,† ·이창문****, *****, ******,†

  • *조선대학교 구강악안면외과학교실, **조선대학교 치의학연구원, ***부경대학교 스마트헬스케어학부,
    ****전남대학교 헬스케어메디컬공학부, *****전남대학교 의공학과, ******전남대학교 헬스케어의공학연구소

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

Abstract

In this study, gallic acid (GA) was conjugated onto chitosan by free radical-mediated covalent binding and the resultant gallic acid-grafted-chitosan (GAC) was prepared in the form of a gel. Compared to the plain chitosan (PC) gel prepared without addition of GA, the wound healing effect of the GAC gel was evaluated in a rat wound model. Both the PC gel and the GAC gel did not show cytotoxicity and the GAC gel effectively inhibited nitric oxide production induced by lipopolysaccharide in RAW264.7 cells. In addition, the GAC gel stimulated more fibroblast migration than the PC gel. In wound model of rats, the GAC gel induced the formation of a normal epidermal layer on the wound site and showed a faster wound healing behavior than the PC gel. These findings suggest that the GAC gel is considered to be useful as a dressing material for wound healing.


본 연구에서는 자유라디칼 매개 공유 결합 형성 방법을 이용하여 gallic acid(GA)를 키토산(chitosan)에 공유 결합하여 제조한 gallic acid-grafted-chitosan(GAC)을 젤(gel) 형태로 제조하여 rat의 창상 모델에서 치유 증진 효과를 평가하였다. 대조구로 GA를 첨가하지 않고 제조한 plain 키토산(PC) 젤을 동일하게 제조하였다. GAC 젤은 PC젤과 유사하게 높은 농도에서 세포독성을 보이지 않았다. GAC 젤은 RAW264.7세포에서 lipopolysaccharide(LPS)에 의해 유도된 nitric oxide(NO)의 생성을 효과적으로 저해하였다. 또한, 섬유아세포의 세포 이동성 평가 결과, GAC 젤은 PC 젤에 비해 세포 이동성을 효과적으로 증진시켰다. Rat의 창상 모델에서 GAC 젤은 창상 부위에 정상 표피층이 잘 형성되도록 유도하였고, PC 젤에 비해 보다 빠른 창상 치유 거동을 보였다. 본 연구를 통해 GA를 첨가하여 제조한 기능성 GAC 젤이 PC 젤에 비해 우수한 NO 생성 저해, 세포 이동성 증진 효과를 통하여 창상부위의 치유를 증진시킨다는 것을 알 수 있었다. 따라서, GAC 젤은 창상 치유를 위한 피복재로써 유용할 것으로 사료된다.


Keywords: chitosan, gallic acid, wound healing, cell migration, nitric oxide release.

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2023; 47(1): 58-63

    Published online Jan 25, 2023

  • 10.7317/pk.2023.47.1.58
  • Received on Sep 21, 2022
  • Revised on Oct 26, 2022
  • Accepted on Oct 27, 2022

Correspondence to

  • Jae-Young Je*** , and Chang-Moon Lee****, *****, ******

  • ***Division of Smart Healthcare, Pukyong National University, Busan 48513, Korea
    ****School of Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea
    *****Department of Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea
    ******Research Center of Healthcare and Biomedical Engineering, Chonnam National University, Yeosu 59626, Korea

  • E-mail: jjy1915@pknu.ac.kr, cmlee@jnu.ac.kr