• Interaction of Fibroblast Cells onto chloric Acid-treated Poly(α-hydroxy acid) Polymer Surfaces
  • Lee SJ, Khang G, Lee JH, Lee YM, Lee HB
  • 염소산 처리된 Poly(α-hydroxy acid)계 고분자 표면과 섬유아세포의 상호작용
  • 이상진, 강길선, 이진호, 이영무, 이해방
Abstract
PLA, PGA and PLGA films were treated with chloric acid mixture solution[70% perchloric acid(HClO4)/potassium chlorate(KClO3) aq. saturated solution, 3:2] to increase surface wettability and thus cell compatibility. The surface-treated PLA, PGA, and PLGA films were characterized by the measurement of water contact angle, electron spectroscopy for chemical analysis, and scanning electron microscopy. Surface wettability of chloric acid-treated PLA, PGA, and PLGA film surfaces was gradually increased with increase of treatment time. Unlike EtOH pre-treatment, chloric acid-treated polymer films maintain hydrophilic surface after drying. In cell adhesion test, fibroblasts were cultured on the chloric acid-treated film surfaces for 1 and 2 days. As the surface wettability increased, the cell adhesion on the surface were increased. In conclusion, this study demonstrated that the surface wettability of polymer plays an important role for cell adhesion and proliferation behavior.

본 연구에서는 폴리락타이드(PLA), 폴리글리콜라이드(PGA) 그리고 이들의 공중합체인 PLGA 필름을 염소산 혼합용액 [70% 과염소산(HClO4)/포타슘 클로레이트(KClO3) 포화수용액,3:2]으로 처리하여 표면의 젖음성과 세포적합성을 증가시켰다. 표면 처리된 고분자의 표면을 물접촉각 측정과 ESCA, SEM으로 특성결정하였다. 염소산 처리된 PLA,PGA 및 PLGA의 표면 젖음성은 처리시간이 증가함에 따라 증가되었고 이들 고분자 필름은 기존의 에탄올 전처리와 달리 건조 후에도 친수성 표면을 유지하였다. 세포 점착실험은 섬유아세포를 염소산 처리된 필름의 표면에서 1일 및 2일 배양하였고 표면의 젖음성이 증가함에 따라 세포의 점착도 우세하였다. 결론적으로 본 연구에서는 표면의 젖음성은 세포의 점착과 증식 거동에 중요한 역할을 한다는 것을 증명하였다.

Keywords: chloric acid mixture solution; surface wettability; cell compatibility; cell adhesion and proliferation behavior

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  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2024 Impact Factor : 0.6
  • Indexed in SCIE

This Article

  • 2000; 24(6): 877-885

    Published online Nov 25, 2000