Article
  • Gellan Gum/Polyvinyl Alcohol Hydrogels with Stress Relaxation Property for Application as Retinal Pigment Epithelial Cell Carrier

  • Seung Ho Choe*,#, Soo In Kim*,#, Se Eun Kim*, Seung Jae Kim*, Jin Sol Seo*, Sunjae Park*, Jeong Eun Song*, and Gilson Khang*, **, ***,†

  • *Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Korea
    **Department of PolymerNano Science & Technology, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Korea
    ***Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Korea

  • 망막색소상피 세포전달체로 응용하기 위한응력 완화 특성을 갖는 젤란검/폴리비닐알코올 하이드로젤

  • 최승호*,# · 김수인*,# · 김세은* · 김승재* · 서진솔* · 박선재* · 송정은* · 강길선*, **, ***,†

  • *전북대학교 바이오나노융합공학과, **전북대학교 고분자나노공학과, ***전북대학교 고분자융합소재연구소

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

Abstract

In this study, the stress relaxation of gellan gum (GG)/polyvinyl alcohol(PVA) hydrogel was evaluated according to the molecular weight of PVA. In addition, survival and proliferation of retinal pigment epithelium (RPE) were evaluated. After mixing GG with three molecular weights of PVA, Ca2+ was added to form a cross-link. The prepared viscoelastic hydrogels were designated as GG/PVA 146, GG/PVA 85, and GG/PVA 31 hydrogels. The physicochemical and mechanical properties of the GG/PVA hydrogel were evaluated, and in vitro experiments were conducted to confirm the effect of the GG/PVA hydrogel on the activity of ARPE-19 cells. As a result of the experiment, as the PVA molecular weight decreased, the hydrogel showed a high-water content, and we confirmed the fast stress relaxation property. In addition, rapid proliferation and spread were observed when ARPE-19 cells were cultured in gel. These results suggest that the applicability of the GG/PVA 31 hydrogel with fast stress relaxation as an ARPE-19 cell delivery system.


본 연구에서는 폴리비닐알코올(polyvinyl alcohol, PVA) 분자량에 따른 젤란검(gellan gum, GG)/PVA 하이드로젤의 응력 완화 평가와 이에 따른 망막색소상피 세포(retinal pigment epithelium, RPE)의 생존 및 증식을 평가하였다. GG를 세가지 분자량의 PVA에 각각 혼합한 후, Ca2+를 첨가하여 가교결합을 형성시켰다. 제조된 점탄성 하이드로젤은 GG/PVA 146, GG/PVA 85, GG/PVA 31 하이드로젤이라 명명하였다. GG/PVA 하이드로젤의 물리화학적 및 기계적 특성을 평가하였고, GG/PVA하이드로젤에서 ARPE-19 세포의 활성에 미치는 영향을 확인하기 위해 in vitro 실험을 하였다. 실험 결과, PVA 분자량이 감소할수록 높은 수분 함유량을 보였으며, 빠른 응력완화 특성을 확인하였다. 또한, 겔내 ARPE-19 세포 배양시 빠른 증식과 확산을 관찰하였다. 이러한 결과를 통해, 빠른 응력완화를 갖는 GG/PVA 31 하이드로젤의ARPE-19 세포전달체로 응용가능성을 확인하였다.


Keywords: gellan gum, polyvinyl alcohol, stress relaxation, 3D cell culture, retinal pigment epithelium.

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2023; 47(2): 233-240

    Published online Mar 25, 2023

  • 10.7317/pk.2023.47.2.233
  • Received on Dec 2, 2022
  • Revised on Jan 26, 2023
  • Accepted on Jan 30, 2023

Correspondence to

  • Gilson Khang

  • *Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Korea
    **Department of PolymerNano Science & Technology, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Korea
    ***Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Korea

  • E-mail: gskhang@jbnu.ac.kr